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324717 Sigma-Aldrich

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli

Endoglycosidase H, Streptomyces plicatus, Recombinant, E. coli cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Synonym: Endo-β-N-acetylglucosaminidase H, Endo H, Endo-β-N-acetylglucosaminidase H, Endo H

  • CAS Number 52769-51-4

  •  EC Index Number 3.2.1.96

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
packaging   200 miu in Plastic ampoule (324717-200MIU)
form   liquid
mfr. no.   Calbiochem
shipped in   wet ice
specific activity activity   ≥3 units/mL
  ≥30 units/mg protein
storage condition   do not freeze
impurities   N-acetylglucosaminidase, α- and β-galactosidase, α-mannosidase, neuraminidases, proteases: none detected
storage conditions   +2C to +8C

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, streptomyces plicatus endoglycosidase H expressed in E. coli. Catalyzes the hydrolysis of asparagine-linked oligomannose and hybrid, but not complex oligosaccharides from glycoprotein. Endo H cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide.

Other Notes

Rao, V., et al. 1995. Structure 3, 449.
Trimble, R.B., et al. 1987. Methods Enzymol. 138, 763.
Trumbly, R.J., et al. 1985. J. Biol. Chem.260, 5683.

Packaging

200 miu in Plastic ampoule

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE.

Warning

Standard Handling (A)

Physical form

In 50 mM NaCl, 20 mM Tris-HCl, 1 mM EDTA, pH 7.5.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
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