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  • 324870 - Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

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324870 Sigma-Aldrich

Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb

liquid, clone, Calbiochem®

Synonym: Anti-α-ENaC

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Properties

Related Categories AL-AL, Alphabetical Index, Antibodies, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   human, rat
form   liquid
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~97 kDa glycosylated α-ENaC protein.

Recognizes the ~97 kDa glycosylated α-ENaC protein.

This Anti-α-Epithelial Cell Sodium Channel (20-42) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of α-Epithelial Cell Sodium Channel (20-42).

Immunogen

Human

a synthetic peptide [LMKGNKREEQGLGPEPAAPQQPT(C)] corresponding to amino acids 20-42 of human α-epithelial cell sodium channel (α-ENaC), conjugated to carrier protein

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see comments)

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Packaging

100 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Other Notes

Benos, D.J., and Stanton, B.A. 1999. J. Physiol. London520, 631.
Mano, I., and Driscoll, M. 1999. Bioessays21, 568.
Fyfe, G.K., et al. 1998. Semin. Nephrol.18, 138.

Does not cross-react with β-ENaC or γ-ENaC. Also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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