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  • 325896 - Wee1, GST-Fusion, Human, Recombinant, S. frugiperda

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325896 Sigma-Aldrich

Wee1, GST-Fusion, Human, Recombinant, S. frugiperda

  •  EC Index Number 2.7.1.112

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Properties

Related Categories Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates More...
brand family   Calbiochem
form   Liquid
shipped in   wet ice
specific activity   ≥1 U/mg protein
storage conditions   = -70C
  Avoid freeze/thaw
  Ok to freeze

Description

General description

Recombinant, human WEE1 fused at the N-terminus to a GST-His6-thrombin cleavage site sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. WEE1 is a tyrosine kinase that causes inhibitory phosporylation of Cdk1 and Cdk2 and acts as a mitotic inhibitor. Protects the nucleus from cytoplasmically activated cyclin B1-complexed CDC2 prior to the onset of mitosis.

Other Notes

Katayama, K., et al. 2005. Mol. Cell. Biol.25, 5725.
Yoshida, T., et al. 2004. Ann. Oncol.15, 252.
Watanabe, N., et al. 1995. EMBO J.14, 1878.
Igarashi, M., et al. 1991. Nature353, 80.

Packaging

Please refer to vial label for lot-specific concentration.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 nmol phosphate to Poly(Ala,Glu,Lys,Tyr)6:2:5:1 per min at 30°C, using variable concentrations of ATP (100 nM to 1.55 µM).

Warning

Standard Handling (A)

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 5 mM DTT, 4 mM reduced glutathione, 20% glycerol, pH 8.0.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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