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341006 Sigma-Aldrich

EDAC, Hydrochloride

EDAC HCl is a water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis.

Synonym: EDAC, Hydrochloride, EDCI, 1-Ethyl-3-(3ʹ-dimethylaminopropyl)carbodiimide, HCl

  • CAS Number 25952-53-8

  • Empirical Formula (Hill Notation) C8H17N3 · xHCl

  • Molecular Weight 155.24 (free base basis)

  •  MDL number MFCD00012503

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Properties

Quality Level   200
assay   ≥98% (titration)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   -20C
color   white
solubility   aqueous buffer: 2-5 mg/mL
  water: 2-5 mg/mL
shipped in   ambient
InChI   1S/C8H17N3.ClH/c1-4-11(8-9)7-5-6-10(2)3;/h4-7H2,1-3H3;1H
InChI key   FDXPUDRRFDHONO-UHFFFAOYSA-N

Description

General description

Molar absorptivity (~212 nm; 50 mM phosphate buffer, pH 8):≥6800 M-1cm-1.

Water-soluble carbodiimide derivative. Used to modify NMDA receptors. Also useful for conjugating haptens to proteins or polypeptides.

Water-soluble derivative of carbodiimide useful for conjugating haptens to proteins and polypeptides. Used to modify NMDA receptors and as a condensing agent in peptide synthesis. The major advantage of EDAC coupling is the easy removal of excess reagent and the corresponding urea by washing with dilute acid or water. Carbodiimides catalyze the formation of amide bonds, carboxylic acids, and amines by activating the carboxylate to form an O-acylurea. This intermediate can be attacked by an amine directly to form an amide. EDAC is released as a soluble urea derivative.

Packaging

25 g in Glass bottle

5 g in Plastic ampoule

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Preparation Note

Avoid amine containing buffers.

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Other Notes

Chazot, P.L., et al. 1993. Biochem. Pharmacol. 45, 605.
Richardson, A., et al. 1992. Biochem. Pharmacol. 43, 1415.
Taniuchi, M., et al. 1986. Proc. Natl. Acad. Sci. USA83, 1950.
Chase, J.W., et al. 1983. Proc. Natl. Acad. Sci. USA80, 5480.
Williams, A., et al. 1981. J. Am. Chem. Soc.103, 7090.
Yamada, H., et al. 1981. Biochemistry20, 4836.
Thomas, J.O., et al. 1978. J. Mol. Biol.123, 149.
Ozawa, H. 1970. Biochemistry9, 2158.
Kopple, K.D., et al. 1962. J. Am. Chem. Soc.84, 4457.

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