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341589 Sigma-Aldrich

FGF, Acidic, Mouse, Recombinant

Synonym: HBGF-1

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Properties

Quality Level   100
assay   ≥95% (PAGE)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   <1.0 EU/μg protein Endotoxin
shipped in   ambient

Description

General description

Recombinant, mouse fibroblast growth factor (FGF) acidic, consisting of amino acids 16-155 and expressed in E. coli, with an N-terminal Met. FGF acidic stimulates proliferation of all cells of mesodermal origin and many cells of ectodermal, neuroectodermal, and endodermal origin. Intracellular FGF acidic inhibits p53 activity and proapoptotic signaling, thereby enabling it to function as a survival factor.

Biochem/physiol Actions

ED₅₀ = 0.05-0.25 ng/ml, in the presence of 10 µg/ml heparin, as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered solution in MOPS, Na₂SO₄, EDTA, DTT, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Guerrini, M., et al. 2007. Curr. Pharm. Des.13, 2045.
Nilsen, T., et al. 2007. J. Biol. Chem.282, 26245.
Rajalingam, D., et al. 2007. Biochemistry46, 9225.
Wesche, J., et al. 2006. J. Biol. Chem.281, 11405.
Bouleau, S., et al. 2005. Oncogene24, 7839.
Mohammadi, M., et al. 2005. Cytokine Growth Factor Rev.16, 107.
Presta, M., et al. 2005. Cytokine Growth Factor Rev.16, 159.
Robinson, C.J., et al. 2005. J. Biol. Chem.280, 42274.
Wesche, J., et al. 2005. Biochemistry44, 6071.
Wiedlocha, A., et al. 2005. Mol. Biol. Cell16, 794.
Wiedlocha, A. and V. Sorensen. 2004. Curr. Top. Microbiol. Immunol.286, 45.
Allen, B.L. and A.C. Rapraeger. 2003. J. Cell Biol.163, 637.
Galzie, Z., et al. 1997. Biochem. Cell Biol.75, 669.
Madiai, F., et al. 1996. Gene179, 231.
Imamura, T., et al. 1990. Science249, 1567.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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