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341590 Sigma-Aldrich

FGF, Basic, Rat, Recombinant

Synonym: HBGF-2

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Properties

Quality Level   100
assay   ≥95% (SDS-PAGE)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   <1.0 EU/μg protein Endotoxin
shipped in   ambient

Description

General description

Recombinant, rat fibroblast growth factor (FGF) basic consisting of amino acids 11-154 and expressed in E. coli. FGF basic, a member of the FGF family of mitogenic peptides, is known to bind heparine or cell surface heparin sulfate proteoglycans for binding, dimerization, and activation of tyrosine kinase FGF receptors. Also known to bind polysaccharides, proteins, and lipids with lower affinity.

Packaging

25 μg in Glass bottle

Biochem/physiol Actions

ED₅₀ = 0.3-1.8 ng/ml as measured in a cell proliferation assay using NR6R-3T3 mouse fibroblasts.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered MOPS, Na₂SO₄, EDTA, DTT, BSA.

Reconstitution

Reconstitute to 100 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.

Other Notes

Presta, M., et al. 2005. Cytokine Growth Factor Rev.16, 159.
Quarto, N., et al. 2005. Gene356, 49.
Tsuneto, M., et al. 2005. Biochem. Biophys. Res. Commun.335, 1239.
Claus, P., et al. 2003. J. Biol. Chem.278, 479.
Coulier, F., et al. 1997. J. Mol. Evol.44, 43.
Fernig, D., et al. 1994. Prog. Growth Factor Res.5, 353.
Shimasaki, S., et al. 1988. Biochem. Biophys. Res. Commun.157, 256.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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