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341635 Sigma-Aldrich

Fibronectin, Human Plasma

Synonym: Fibronectin, Human Plasma

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Properties

Quality Level   200
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C
shipped in   wet ice

Description

General description

Native fibronectin purified from human plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

Packaging

1, 5 mg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.3.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Thaw carefully using the guidelines outlined above. Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Contaminants: none detected by IEP with antiserum to fibronectin or normal human serum

single major band by SDS-PAGE

Other Notes

Akiyama, S.K., and Yamada, K.M. 1987. Adv. Enzymol.59, 1.
Krejci, K., and Fritz, H. 1976. FEBS Lett.64, 152.


Selected Citations
Hoffmeister, M., et al. 2008. J. Biol. Chem.283, 2297.


Brown, S., et al. 2002. Nature418, 200.


Kim, M., FEBS Letters581 1917.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

Peer-Reviewed Papers
15

References

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