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341668 Sigma-Aldrich

Fibronectin, Rat Plasma

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Properties

Related Categories Biochemicals and Reagents, Cell Culture, Enzymes, Inhibitors, and Substrates, Reagents and Supplements
form   liquid
mfr. no.   Calbiochem
shipped in   dry ice
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
purity   single major band by SDS-PAGE
impurities   None detected by IEP using anti-fibronectin and anti-rat serum
storage conditions   -20C

Description

General description

Native fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.

Packaging

500 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.

Warning

Standard Handling (A)

Physical form

In 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Extracellular Matrix Proteins and tools for cell culture optimization

Animal cells and tissue culture techniques are constantly improved to optimize in vitro cell culture conditions. Extracellular Matrix (ECM) proteins coating, chemical or physical modification of the ...
Keywords: Adhesion, Angiogenesis, Apoptosis, Asymmetric synthesis, Cancer, Cell attachment, Cell culture, Cell proliferation, Coagulation, Endocrinology, Growth factors, Hormones

HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Statins in Rat Plasma on Ascentis® Express C18 after SPE using Supel™-Select HLB
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Sample preparations, Solid phase extractions

HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis® Express C18 after Phospholipid Removal using HybridSPE®-Phospholipid
Keywords: Antihypertensives, Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Pharmaceutical, Precipitation, Sample preparations, Solid phase extractions

LC/MS Analysis of Clenbuterol In Plasma on Astec® CHIROBIOTIC® T with Phospholipid Removal using HybridSPE® Phospholipid

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Clenbuterol In Plasma on Astec® CHIROBIOTIC® T with Phospholipid Removal using HybridSPE® Phospholipid
Keywords: Chromatography, Clinical, Forensic, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Precipitation, Sample preparations, Solid phase extractions

LC/MS Analysis of Plasma Samples on Ascentis® Express C18 Showing Baseline Improvements after Phospholipid Removal using HybridSPE®-Phospholipid

From our library of Articles, Sigma-Aldrich presents LC/MS Analysis of Plasma Samples on Ascentis® Express C18 Showing Baseline Improvements after Phospholipid Removal using HybridSPE®-Phospholipid
Keywords: Chromatography, Clinical, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Pharmaceutical, Precipitation, Sample preparations, Solid phase extractions

What is the Extracellular Matrix?

The extracellular matrix (ECM) is secreted by cells and surrounds them in tissues. It has long been understood to be the structural support for cells since its characteristics set the characteristics...
Keywords: Adhesion, Cell culture, Cellular processes, Epimerizations, Growth factors, Immobilization, Sulfations

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