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344289 Sigma-Aldrich

Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum

This Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum is validated for use in Immunization.

Synonym: Freund’s Complete Adjuvant, Modified, Mycobacterium butyricum



Quality Level   100
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   clear to slightly cloudy amber
shipped in   ambient


General description

1 Set (10 x 10 ml) each containing yellow, oily, bacterial suspension.

Contains 10 vials per set, each containing 10 ml of Freund′s complete adjuvant homogenized in the following proportions by weight: 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier), and 0.1% Mycobacterium butyricum dry cells.


1 set in Glass bottle




Toxicity: Standard Handling (A)

Physical form

Yellow, oily, bacterial suspension.

Other Notes


The Freund′s Adjuvant set contains 10 identical ampules, each with 10 ml of Freund′s Complete Adjuvant homogenized in the following preparations by weight:

85% Drakeol 5 NF

15% Arlacel A, mannide monooleate emulsifier

0.1% Mycobacterium butyricum dry cells

Sample Preparation

1. Withdraw contents of ampule using a 10 ml syringe with a 1 inch 21 guage needle. Add to a 50 ml vial, preferably with a narrow neck.

2. Dissolve 50 mg of antigen in 5 ml sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible. Draw the solution into the syringe.

3. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.

4.Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Mixture may seem to thicken as the emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
WGK Germany 
Protocols & Articles
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