• USA Home
  • 345810 - G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

EMAIL THIS PAGE TO A FRIEND
345810 Millipore

G 418 Sulfate, Cell Culture Tested - CAS 108321-42-2 - Calbiochem

G418 also known as Geneticin® is an aminoglycoside antibiotic related to Gentamicin. Used as a selective agent in transfection of eukaryotic cells. Has highest potency ≥730 µg/mg and purity ≥98%.

Synonym: G418, G 418, NMD Inhibitor I

  • CAS Number 108321-42-2

  • Linear Formula C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

Purchase

Properties

Related Categories Antibiotics, Bioactive Small Molecules, Biochemicals and Reagents, Cell Biology, Cell Culture,
brand family   Calbiochem
packaging   1 gm in Glass bottle (345810-1GM)
  100 gm in Glass bottle (345810-100GM)
  25 gm in Plastic ampoule (345810-25GM)
  250 mg in Plastic ampoule (345810-250MG)
  5 gm in Glass bottle (345810-5GM)
  500 mg in Plastic ampoule (345810-500MG)
form   White powder
purity   ≥98% by TLC
impurities   Ammonia: ≤1.0%; heavy metals: ≤100 ppm
toxicity   Standard Handling
shipped in   ambient
storage temp.   +15C to +30C
storage conditions   Ok to freeze

Description

Overview

Also available as a sterile-filtered aqueous solution (Cat. No. 345812), a sterile filtered solution in 100 mM HEPES (Cat. No. 508940), and a sterile-filtered solution PBS (Cat. No. 509227).

Aminoglycoside antibiotic related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis, is toxic to bacteria, yeast, protozoans, helminths, higher plant and mammalian cells. Used in molecular genetics as a selective agent for the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3'-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Aminoglycoside related to gentamycin that inhibits prokaryotic and eukaryotic protein synthesis. Toxic to bacteria, yeast, protozoans, helminths, higher plant, and mammalian cells. Widely used in the selection of eukaryotic expression vectors carrying the bacterial neor/kanr genes. The product of these genes, aminoglycoside 3′-phosphotransferase, inactivates G418, neomycin, and kanamycin by phosphorylation. Introduction of either of these genes into cells can confer resistance to G418, which enables cells to grow in media containing G418.

Biological Activity

Assay: ≥730 µg/mg

Storage and Shipping Instructions

Following reconstitution, sterilize by filtration through a 0.22 µm or 0.45 µm pore size filter, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Sterile stock solutions are stable for up to 1 year at -20°C.

Safety & Documentation

Safety Information

RTECS 
CB9378500
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?