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36-002 Sigma-Aldrich

Anti-phospho-SGK Antibody

Anti-phospho-SGK Antibody is an antibody against phospho-SGK for use in WB.

  •  eCl@ss 32160702

  •  NACRES NA.41

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Properties

Related Categories Alphabetical Index, Antibodies, Primary Antibodies, SG-SK
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   human
shipped in   dry ice
antibody form   serum
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
mfr. no.   Upstate®
NCBI accession no.   NM_005627.2
UniProt accession no.   O00141
Gene Information   human ... SGK1(6446)

Description

Specificity

Recognizes phospho-SGK.

Immunogen

peptide corresponding to amino acids surrounding Ser255/Thr256 of human SGK1.

Application

Not recommended for use with cell lysates.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Target description

48 kDa

Physical form

UnPurified whole rabbit antiSerum containing 30% glycerol and 0.035% sodium azide.

Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Quality

routinely evaluated by immunoblot detected phospho-SGK after phosphorylation of unactive SGK1 (Catalog # 14-332) with PDK1 (Catalog # 14-280)

Analysis Note

Control
PDK activated SGK samples

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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