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  • 361527 - PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

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361527 Sigma-Aldrich

PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

liquid, clone 2D3, Calbiochem®

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Properties

Related Categories Alphabetical Index, Antibodies, GQ-GZ, Primary Antibodies
clone   2D3, monoclonal
biological source   mouse
species reactivity   mouse, human
form   liquid
shipped in   wet ice
isotype   IgG1
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative (antibody only)
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C

Description

General description

Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.

Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.

This PhosphoDetect Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

Immunogen

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β

Application

ELISA (0.05 µg/ml)

Immunoblotting (0.5 µg/ml)

Physical form

100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.

Reconstitution

Reconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.

Packaging

1 set in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Pervanadate-treated HepG2 (included)

Other Notes

For immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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