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361550 Sigma-Aldrich

GSK-3 Inhibitor IX - CAS 667463-62-9 - Calbiochem

BIO, CAS 667463-62-9, is a cell-permeable, highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC₅₀ = 5 nM). Maintains self-renewal in human & mouse embryonic stem cells.

Synonym: BIO, (2ʹZ,3ʹE)-6-Bromoindirubin-3ʹ-oxime

  • CAS Number 667463-62-9

  • Linear Formula C₁₆H₁₀BrN₃O₂

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Properties

Related Categories Biochemicals and Reagents, D to K, Enzyme Inhibitors, Enzyme Inhibitors by Enzyme, Enzymes, Inhibitors, and Substrates,
brand family   Calbiochem
packaging   1 mg in Plastic ampoule (361550-1MG)
  10 mg in Plastic ampoule (361550-10MG)
  30 mg in Plastic ampoule (361550-30MG)
ATP competitive   Y
form   Purple solid
reversible   Y
primary target   GSK-3α/β
primary target IC50   5 nM against GSK-3α/β
purity   ≥97% by HPLC
cell permeable   Y
toxicity   Carcinogenic / Teratogenic
shipped in   wet ice
storage temp.   +2C to +8C
storage conditions   Protect from light
  Ok to freeze

Description

Overview

A cell-permeable bis-indolo (indirubin) compound that acts as a highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC50 = 5 nM). Its specificity has been tested against various Cdk's (IC50 = 83, 300, 320, and 10,000 nM for Cdk5/p25, Cdk2/A, Cdk1/B, and Cdk4/D1, respectively) as well as many other commonly studied kinases (IC50 ≥ 10 µM), including MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK. Inhibition of GSK by BIO has been shown to result in the activation of Wnt-signaling pathway and sustained pluripotency in human and murine ESCs (embryonic stem cells). Reported to maintain self-renewal in human and mouse embryonic stem cells. Also induces the differentiation of neonatal cardiomyocytes. A 10 mM (500 µg/140 µl) solution of GSK-3 Inhibitor IX (Cat. No. 361552) in DMSO is also available.

A cell-permeable, highly potent, selective, reversible, and ATP-competitive inhibitor of GSK-3α/β (IC50 = 5 nM). Specificity was confirmed using various Cdk's (IC50 = 83 nM for Cdk5/p25, 300 nM for Cdk2/cyclin A, 320 nM for Cdk1/cyclin B, and 10 µM for Cdk4/cyclin D1), MAP kinases, PKA, PKC isoforms, PKG, CK, and IRTK (IC50 ≥10 µM). Inhibition of GSK by BIO has been shown to result in the activation of the Wnt signaling pathway and sustained pluripotency of human and murine embryonic stem cells (ESCs). Reported to maintain self-renewal in human and mouse embryonic stem cells. Also induces the differentiation of neonatal cardiomyocytes.

Packaging

Packaged under inert gas

Storage and Shipping Instructions

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Safety & Documentation

Safety Information

R Phrase 
R: 20/21/22

Harmful by inhalation, in contact with skin and if swallowed.
S Phrase 
S: 22-36/37

Do not breathe dust.
Wear suitable protective clothing and gloves.
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