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371206 Sigma-Aldrich

Dkk-1, His•Tag , Mouse, Recombinant

Dkk-1, His•Tag®, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells.

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Cycle Proteins, Antibodies and Reagents, Cell Cycle Regulators, Cell Signaling and Neuroscience More...
assay   ≥95% (SDS-PAGE)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   ≤1.0 EU/μg protein Endotoxin
shipped in   ambient

Description

General description

Dkk-1, His•Tag, Mouse, Recombinant, consists of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells.

Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.

Packaging

10 μg in Glass bottle

Biochem/physiol Actions

ND₅₀: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells.

Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos.

Warning

Toxicity: Regulatory Review (Z)

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Other Notes

Pinzone, J.J. et al. 2009. Blood113, 517.
Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Glinka, A. et al. 1998. Nature391, 357.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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