• USA Home
  • 371208 - Dkk-1, Rat, Recombinant

EMAIL THIS PAGE TO A FRIEND
371208 Sigma-Aldrich

Dkk-1, Rat, Recombinant

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

Purchase

Properties

Quality Level   100
assay   ≥95% (SDS-PAGE)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   ≤1.0 EU/μg protein Endotoxin
shipped in   ambient

Description

General description

Dkk-1, Rat, Recombinant, consists of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells.

Recombinant, rat Dickkopf related protein-1 (Dkk-1), consisting of amino acids 23-270, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. The balance between Wnt signaling and Dkk-1 inhibition is essential for bone formation and homeostasis.

Packaging

10 μg in Glass bottle

Biochem/physiol Actions

ND₅₀ = 0.1-0.5 µg/ml as measured by inhibition of Wnt-3a-induced (10 ng/ml rmWnt-3a) alkaline phosphatase production by proliferation of MC3T3-E1 mouse osteoblastic cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered PBS, BSA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.

Other Notes

Li, J. et al. 2006. Bone36, 754.
Morvan, F. et al. 2006. J. Bone Miner. Res.21, 934.
Niehrs, C. 2006. Oncogene25, 7469.
Verani, R. et al. 2006. J. Neurochem.101, 242.
Cappuccio, I. et al. 2005. J. Neurosci.25, 2647.
Kemp, C. et al. 2005. Dev. Dyn.233, 1064.
Adamska, M. et al. 2004. Dev. Biol.272, 134.
Bullock, C.M. et al. 2004. Mol. Pharmacol.65, 582.
Mao, B. et al. 2002. Nature417, 664.
Mao, B. et al. 2001. Nature411, 321.
Krupnik, V.E., et al. 1999. Gene238, 301.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?