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  • 371723 - Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb

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371723 Sigma-Aldrich

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355) Rabbit pAb

Anti-Giα-1 and Giα-2-Subunits, C-Terminal (345-354 and 346-355), rabbit polyclonal, recognizes Giα-1 and Giα-2 subunits. It is validated for use in Western blotting.

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Properties

Related Categories Alphabetical Index, Antibodies, G-GC, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity (predicted by homology)   mammals
form   liquid
shipped in   wet ice
isotype   IgG
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C

Description

General description

Protein A purified rabbit polyclonal antibody. Recognizes Giα-1 and Giα-2 subunit proteins.

Recognizes Giα-1 and Giα-2 subunits. Does not cross-react with G3α-3 or Goα subunits.

Immunogen

a synthetic peptide [(C)KNNLKDCGLF] corresponding to amino acids at the C-terminus of mammalian Giα-1 and Giα-2, conjugated to KLH

Application

Immunoblotting (1:1000)

Physical form

In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Packaging

50 ul in Glass bottle

Warning

Toxicity: Standard Handling (A)

Other Notes

Does not cross-react with Giα-3 and Goα. The specificity and cross-reactivity were confirmed with lysates from separate cultures of bacteria transfected with the genes for Giα-1, Giα-2, Giα-3, and Goα. Variables associated with assay conditions will dictate the proper working dilution.

Mumby, S.M., and Gilman, A.G. 1991. Methods Enzymol.195, 215.
Goldsmith, P.P., et al. 1987. J. Biol. Chem.262, 14683.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

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