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371818 Sigma-Aldrich

G-Protein βγ Binding Peptide, mSIRK - Calbiochem

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange.

Synonym: mSIRK, myr-SIRKALNILGYPDYD-OH, G-Protein βγ Binding Peptide, mSIRK - Calbiochem

  • Empirical Formula (Hill Notation) C93H150N20O25

  • Molecular Weight 1948.31

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Properties

Quality Level   100
assay   ≥95% (HPLC)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   +2C to +8C
solubility   DMSO: 5 mg/mL
shipped in   ambient

Description

General description

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes α subunit dissociation without stimulating nucleotide exchange. Reported to cause a rapid activation of ERK1/2 (EC50 ~2.5-5 µM) in RASM (rat arterial smooth muscle) and Rat2 cells. Promotes the phosphorylation of JNK and p38 MAP kinase. Also increases phospholipase C activity and causes Ca2+ release from internal stores.

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC50 ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca2+ release from internal stores. Negative control is also available (Cat. No. 371820).

Packaging

1, 5 mg in Glass bottle

Biochem/physiol Actions

Cell permeable: yes

EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells

Primary Target
N-myristoylated G-protein βγ selective binding

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

Myr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis (COA)

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