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  • 373270 - Hec1/Nek2 Mitotic Pathway Inhibitor I, INH1 - CAS 313553-47-8 - Calbiochem

373270 Sigma-Aldrich

Hec1/Nek2 Mitotic Pathway Inhibitor I, INH1 - CAS 313553-47-8 - Calbiochem

The Hec1/Nek2 Mitotic Pathway Inhibitor I, INH1, also referenced under CAS 313553-47-8, controls the biological activity of Hec1/Nek2 Mitotic Pathway.

Synonym: Inhibitor of Nek2/Hec1-1, N-(4-(2,4-Dimethylphenyl)-thiazol-2-yl)-benzamide, INH1, Hec1/Nek2 Mitotic Pathway Inhibitor I, INH1 - CAS 313553-47-8 - Calbiochem, N-(4-(2,4-Dimethylphenyl)-thiazol-2-yl)-benzamide, INH1, Inhibitor of Nek2/Hec1-1

  • CAS Number 313553-47-8

  • Empirical Formula (Hill Notation) C18H16N2OS

  • Molecular Weight 308.40



Quality Level   100
assay   ≥97% (HPLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   +2C to +8C
color   white
solubility   DMSO: 50 mg/mL
  ethanol: 50 mg/mL
shipped in   ambient


General description

A cell-permeable thiazolyl benzamide compound that directly binds Hec1 and disrupts Hec1/Nek2 complex formation, resulting in defective Hec1 kinetochores localization and cellular Nek2 destabilization/downregulation. Shown to induce a transient mitotic arrest in HeLa cultures and inhibit the proliferation of 10 breast cancer cell lines (GI50 of 10-21 µM) as well as the cervical HeLa and colon HCT116 cancer lines, while exhibiting reduced potency against the noncancerous MCF10A line (GI50 = 41 µM). Reported to suppress the MDA-MB-468-derived tumor growth (by ~53% and ~75% 3 wks after a 7-wk treatment period with 50 mg/kg and 100 mg/kg INH1, repectively, i.p.) in a murine breast cancer model in vivo.


Packaged under inert gas


Toxicity: Standard Handling (A)


Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Wu, G., et al. 2008. Cancer Res.68, 8393.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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