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374805 Sigma-Aldrich

Hemocyanin, Keyhole Limpet, Megathura crenulata

Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.

Synonym: Hemocyanin, Keyhole Limpet, Megathura crenulata, KLH



Quality Level   200
assay   ≥90% (size exclusion chromatography)
form   lyophilized solid
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   blue to blue-gray
shipped in   ambient


General description

Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3

Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3

Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.


1 g in Plastic ampoule

250 mg in Plastic ampoule


Toxicity: Standard Handling (A)

Physical form

Lyophilized from BES buffer, MgSO₄.


Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).

Other Notes

Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37.
Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137.
Erlanger, B.F. 1980. Methods Enzymol.70, 85.

Protein concentration: ≥60%

Copper: ≥0.09%

Copper to Protein ratio: ≥1.5 x 10-3

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CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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