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391334 Millipore

BES, Free Acid, ULTROL Grade - CAS 10191-18-1 - Calbiochem

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.

Synonym: BES, Free Acid, ULTROL Grade - CAS 10191-18-1 - Calbiochem, N,N- bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

  • CAS Number 10191-18-1

  • Empirical Formula (Hill Notation) C6H15NO5S

  • Molecular Weight 213.25

  •  MDL number MFCD00007533

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Properties

Related Categories Loading Buffer and Tracking Dyes, Molecular Biology, Nucleic Acid Electrophoresis More...
Quality Level   100
assay   ≥99% dry basis (titration)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +15C to +30C
color   white
pKa (20 °C)   7.12
solubility   water: 1 mg/mL
cation traces   heavy metals: ≤1 ppm
shipped in   ambient
InChI   1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
InChI key   AJTVSSFTXWNIRG-UHFFFAOYSA-N

Description

General description

Absorbance (100 mM, 260 nm):≤0.05.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.

Packaging

100 g in Glass bottle

25 g in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.

Other Notes

De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
Chen, C.A., and Okayama, H. 1988. Biotechniques6, 632.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

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