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391338 Millipore

HEPES, Free Acid, ULTROL Grade - CAS 7365-45-9 - Calbiochem

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C. Absorbance (1.0 M, H2O, 260 nm):≤0.05.

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, HEPES, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  MDL number MFCD00006158

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Properties

Quality Level   100
assay   ≥99% dry basis (titration)
form   crystalline powder
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +15C to +30C
color   white
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   water: 1 M
anion traces   chloride (Cl-): ≤0.01%
  sulfate (SO42-): ≤0.05%
cation traces   Na: ≤0.1%
  heavy metals: ≤1 ppm
shipped in   ambient
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.

A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

Absorbance (1.0 M, H2O, 260 nm):≤0.05.

Absorbance (1.0 M, H2O, 280 nm): ≤0.04

Packaging

1 kg in Glass bottle

25 g in Plastic ampoule

100, 500 g in Glass bottle

5 kg in Fibre carton

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Buffers - From Bnech to Bulk
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