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Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells.

Synonym: Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

  • CAS Number 31282-04-9

  • Empirical Formula (Hill Notation) C20H37N3O13

  • Molecular Weight 527.52

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Properties

assay   ≥90% (HPLC and TLC)
form   lyophilized solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
color   light brown
solubility   distilled water: ≥100 mg/mL
shipped in   ambient

Description

General description

Hygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).



Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).



The analytical data listed below will vary from lot to lot.



Appearance: Off-white to light brown solid

Bioassay: 1000 U/mg

Solubility: ≥100 mg/ml in H2O

Purity (HPLC): ≥90%

Cytotoxicity: Tested in HeLa and/or CHO cells

Potency: ≥1000 µ/mg solid.

Unique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.

Packaging

1 gm in Glass bottle

100 mg in Alu drum

5 gm in Alu drum

500 mg in Alu drum

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Other Notes

Dean, N. 1995. Proc. Natl. Acad. Sci. USA92, 1287.
Hubbard, S. C., et al. 1994. J. Biol. Chem.269, 3717.
Rikkerink, E. H., et al. 1994. Current Genetics25, 202.
Sugimoto, K., et al. 1994. Plant J.5, 863.
MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol.276, 67.
Bilang, R., et al. 1991. Gene100, 247.
Lee, M.G-S., and Van der Ploeg, L. 1991. Gene105, 255.
Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol.57, 1423.
MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother.35, 2125.
Zhou, J., et al. 1991. Gene107, 307.
Bulte, L., and Bennoun, P. 1990. Current Genetics18, 155.
Giordano, T.J., and McAllister, W.T. 1990. Gene88, 285.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.24, 1915.
Carrasco, L., et al. 1989. Pharmacol. Ther.9, 311.
Crespi, C.L., et al. 1989. Carcinogenesis10, 295.
Damm, B., et al. 1989. Mol. Gen. Genetics217, 6.
Egelhoff, T.T., et al. 1989. Mol. Cell. Biol.9, 1965.
Kronstad, J.W., et al. 1989. Gene79, 97.
Perlin, D.S., et al. 1988. J. Biol. Chem.263, 118.
Cullen, D., et al. 1987. Gene57, 21.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.
Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun.117, 6.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Provided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.

Safety & Documentation

Safety Information

RTECS 
WK2130000

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

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