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Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

Synonym: Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

  • CAS Number 31282-04-9

  • Empirical Formula (Hill Notation) C20H37N3O13

  • Molecular Weight 527.52

  •  MDL number MFCD06795479

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Properties

Quality Level   100
assay   ≥90% (HPLC)
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
shipped in   ambient
storage temp.   2-8°C
InChI   1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
InChI key   GRRNUXAQVGOGFE-KPBUCVLVSA-N

Description

General description

Hygromycin B is an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.

Packaging

20, 5 ml in Alu drum

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Physical form

50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Sugimoto, K., et al. 1994. Plant J.5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.34, 1915.
Cullen, D., et al. 1987. Gene57, 21.
Santerre, R.F., et al. 1984. Gene30, 147.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
WGK Germany 
WGK 3
Protocols & Articles

Articles

Antibiotic Kill Curve

Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic to determine the minimum concentration of an antibiotic that c...
Keywords: Antibiotics, Cell culture, Evaporation, Transfection

Peer-Reviewed Papers
15

References

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