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400064 Sigma-Aldrich

Anti-Glucose Transporter-4 Rabbit pAb

liquid, clone, Calbiochem®

Synonym: Anti-Glut-4

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Properties

Related Categories Alphabetical Index, Antibodies, GD-GL, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   mouse, rat, human
form   liquid
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Anti-Glucose Transporter-4, rabbit polyclonal, recognizes the ~40-43 kDa GLUT-4. It is validated for use in ELISA, Western blotting, immunoprecipitation, and immunohistochemistry on frozen sections.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.

Recognizes the ~40-43 kDa GLUT-4 protein.

Immunogen

Mouse

a synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH

Application

ELISA (0.5-1 µg/ml)

Frozen Sections (2-20 µg/ml)

Immunoblotting (1-10 µg/ml; see comments)

Immunoprecipitation (1-10 µg/100 µg tissue membrane)

Physical form

In PBS, 0.1% BSA, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Packaging

50 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Other Notes

Does not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.

Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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