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401152 Sigma-Aldrich

Human IgE, Myeloma

This Human IgE, Myeloma is validated for use as an Immunoassay standard.

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Properties

Related Categories Antibody Characterization and Analysis, Biochemicals and Reagents, Isotype Controls, Proteins and Derivatives
Quality Level   200
form   liquid
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C
isotype   IgE
shipped in   wet ice

Description

General description

Native IgE from human myeloma plasma. Human immunoglobulin E (IgE) purified from the plasma of a myeloma donor. The isotype of the light chain (kappa or lambda) will vary from batch to batch depending on the starting material.

Native IgE from human myeloma plasma.

Packaging

100 μg in Plastic ampoule

Application

Immunoassay standard

Warning

Toxicity: Standard Handling (A)

Physical form

In 150 mM NaCl, 15 mM potassium phosphate buffer, pH 7.4.

Preparation Note

Prepared from plasma that was shown by certified tests to be nonreactive for HBsAg and for antibodies to HCV, HBc, HIV-1 and HIV-2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Single arc by immunoelectrophoresis against antiserum to whole human serum and human IgE

Other Notes

Note: the heavy chain of myeloma IgE may appear as either a single or double band on gel electrophoresis. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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