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  • 401459 - Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

401459 Sigma-Aldrich

Mouse Anti-Human IgG₁, Fc Fragment Specific Alkaline Phosphatase Conjugate

liquid, clone, Calbiochem®



Related Categories Alkaline Phosphatase, Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   monoclonal
form   liquid
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
isotype   IgG
shipped in   wet ice


General description

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG1, Fc fragment.

This Mouse Anti-Human IgG₁, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay, Immunoelectrophoresis for the detection of Human IgG₁.


250 μg in Plastic ampoule


ELISA (see comments)

Enzyme Immunoassay (≥1:5000)

Immunoelectrophoresis (see comments)


Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Other Notes

Monospecific for human IgG1 Fc fragment as determined by immunoelectrophoresis and direct ELISA against normal human serum and normal human IgG. p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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