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  • 401462 - Mouse Anti-Human IgG₂, Fc Fragment Specific Alkaline Phosphatase Conjugate

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401462 Sigma-Aldrich

Mouse Anti-Human IgG₂, Fc Fragment Specific Alkaline Phosphatase Conjugate

liquid, clone, Calbiochem®

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Properties

Related Categories Alkaline Phosphatase, Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   monoclonal
form   liquid
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
isotype   IgG
shipped in   wet ice

Description

General description

Protein A purified mouse monoclonal antibody conjugated to alkaline phosphatase. Recognizes human IgG2 Fc fragment.

This Mouse Anti-Human IgG₂, Fc Fragment Specific Alk-Phos Conjugate is validated for use in ELISA, Enzyme Immunoassay for the detection of Human IgG₂, Fc Fragment Specific.

Packaging

250 μg in Plastic ampoule

Application

ELISA (≥1:5000)

Enzyme Immunoassay (1:5000)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris-HCl, 1 mM MgCl₂, 50% glycerol, pH 8.0.

Other Notes

p-Nitrophenylphosphate, 1 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate for testing immunoenzyme reactivity. Monospecific for human IgG2 Fc fragment as determined by ELISA against normal human serum. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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