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407254 Sigma-Aldrich

IGFBP-3, CF, Mouse, Recombinant

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Properties

Quality Level   100
assay   ≥95% (SDS-PAGE)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   <1.0 EU/μg protein Endotoxin
shipped in   ambient

Description

General description

Recombinant, mouse insulin-like growth factor binding protein 3 (IGFBP-3) consisting of amino acids 22-291 and expressed in NS0-derived murine myeloma cells with four substitutions: Arg250Gln, Gln259Arg, Ser260Gly, Arg271Pro. Mouse IGFBP-3 cDNA encodes a 291 amino acid residue precursor protein with a putative 27 aa residue signal peptide.

Packaging

25 μg in Glass bottle

Biochem/physiol Actions

ED₅₀ = 0.125-0.5 µg/ml as measured by inhibition of the biological activity of IGF-I or IGF-II (30 ng/ml) on MCF-7 human breast adenocarcinoma cells.

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm filtered Acetonitrile, TFA.

Reconstitution

Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.

Reconstitute to 100 µg/ml in sterile PBS.

Other Notes

Schuller, A.G.P., et al. 1994. Mol. Cell. Endoc.104, 57.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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