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  • 407700 - Interleukin-10, Human, Recombinant, S. frugiperda

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407700 Sigma-Aldrich

Interleukin-10, Human, Recombinant, S. frugiperda

Synonym: rhIL-1αrhIL-1βrhIL-10

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Properties

Related Categories Auxiliary Proteins, Biochemicals and Reagents, Other, Proteins and Derivatives
Quality Level   100
assay   ≥95% (SDS-PAGE)
form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   = -70C
impurities   <100 pg/μg Endotoxin (pg/μg IL-10)
shipped in   ambient

Description

General description

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Blocks lipopolysaccharide induction of MMP-2 and MMP-9. Known to reduce neuronal degeneration following CNS injury by blocking glutamate-induced caspase-3 activation. Human IL-10 is also active on murine cell lines.

Recombinant, human interleukin-10 expressed in S. frugiperda insect cells. Pleiotropic cytokine that can exert either immunosuppressive or immunostimulatory effects on a variety of cell types. Potent modulator of monocyte/macrophage function. Exhibits anti-inflammatory effects by suppressing macrophage proliferation. Human IL-10 has cross-species reactivity and is active in mouse cell lines.

Packaging

5 μg in Plastic ampoule

Biochem/physiol Actions

ED₅₀ = 0.2-1 ng/ml as measured in a cell proliferation assay using a mouse mast cell line, MC/9.

Warning

Toxicity: Standard Handling (A)

Physical form

Carrier-free.

Reconstitution

Following reconstitution, aliquot and freeze -70°C. Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.

Reconstitute in sterile water or buffer to a final concentration of 0.1-1 mg/ml as stock solution. Further dilute in buffer such as PBS, 1% BSA.

Other Notes

Bachis, A., et al. 2001. J. Neurosci.21, 3104.
Fortunato, S.J., et al. 2001. Obstet. Gynecol.98, 284.
Fleming, S.D., et al. 1996. J. Immunol. 156, 1143.
Wang, C.Q., et al. 1996. J. Cell Physiol. 166, 305.
Niro, H., et al. 1994. Int. Immunol. 6, 661.
Moore, K., et al. 1993. Annu. Rev. Immunol. 11, 165.
Vieira, P., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 1172.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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