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411483 Sigma-Aldrich

Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate

liquid, clone HP6047, Calbiochem®

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Properties

Related Categories Antibodies, Biotin Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   HP6047, monoclonal
form   liquid
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C
isotype   IgG3
shipped in   ambient

Description

General description

Purified mouse monoclonal antibody conjugated to biotin. Recognizes human IgG3 hinge region.

This Mouse Anti-Human IgG₃, Hinge Specific (HP6047) Biotin Conjugate is validated for use in ELISA for the detection of Human IgG₃, Hinge Specific.

Immunogen

Human

Packaging

200 μg in Plastic ampoule

Application

ELISA (1:500-1:2000, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS, 1% BSA, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Biotinylated with hydroxysuccinimide ester biotin. This biotin-labeled antibody is an extremely versatile reagent because of the high binding affinity for avidin and avidin-labeled compounds. Variables associated with assay conditions will dictate the proper working dilution.



Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: ≤0.1%

Human IgG2: ≤1%

Human IgG3: 100%

Human IgG4: ≤0.1%

Human IgA: ≤0.1%

Human IgE: ≤0.1%

Human IgM: ≤0.1%



Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.001%

Human IgG2: <0.001%

Human IgG3: 100%

Human IgG4: <0.001%

Human IgA: <0.001%

Human IgE: <0.001%

Human IgM: <0.001%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Hamilton, R.G. 1987. Clin. Chem.33, 1707.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

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