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  • 411492 - Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)

411492 Sigma-Aldrich

Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025)

liquid, clone HP6025, Calbiochem®



Related Categories Antibodies, Human IgG Secondary Antibodies and Conjugates, Secondaries listed by Conjugate, Secondaries listed by Target Species, Secondary Antibodies,
Quality Level   100
biological source   mouse
antibody product type   secondary antibodies
clone   HP6025, monoclonal
form   liquid
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C
isotype   IgG
shipped in   wet ice


General description

Purified mouse monoclonal antibody. Recognizes human IgG4 Fc fragment.

This Mouse Anti-Human IgG₄, Fc Fragment Specific (HP6025) is validated for use in Enzyme Immunoassay for the detection of Human IgG₄, Fc Fragment Specific.


400 μg in Plastic ampoule


Enzyme Immunoassay (10 µg/ml, see application references)


Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Physical form

In PBS, pH 7.4.


Following initial thaw, aliquot and freeze (-20°C). Avoid more than 5 freeze/thaw cycles.

Other Notes

Antibody should be titrated for optimal results in individual systems.

Cross-reactivity by ELISA against human myeloma proteins:

Human IgG1: ≤0.1%

Human IgG2: ≤0.04%

Human IgG3: ≤0.02%

Human IgG4: 100%

Human IgA: <0.1%

Human IgE: <0.1%

Human IgM: <0.1%

Cross-reactivity by ELISA against chimeric antibodies:

Human IgG1: <0.01%

Human IgG2: <0.01%

Human IgG3: <0.01%

Human IgG4: 100%

Human IgA: <0.01%

Human IgE: <0.01%

Human IgM: <0.01%

Hamilton, R.G. 1990. Ann. Biol. Clin.48, 473.
Fasullo, F.J., et al. 1989. Clin. Chem.35, 364.
Jefferis, R., et al. 1986. Immunol. Letters10, 223.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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