• USA Home
  • 420130 - JNK Inhibitor III, Cell-Permeable - Calbiochem

EMAIL THIS PAGE TO A FRIEND
420130 Millipore

JNK Inhibitor III, Cell-Permeable - Calbiochem

The JNK Inhibitor III, Cell-Permeable controls the biological activity of JNK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Synonym: HIV-TAT₄₇₋₅₇-gaba-c-Junδ₃₃₋₅₇, Ac-YGRKKRRQRRR-gaba-ILKQSMTLNLADPVGSLKPHLRAKN-NH₂, SAPK Inhibitor III, SAPK Inhibitor III, HIV-TAT₄₇₋₅₇-gaba-c-Junδ₃₃₋₅₇, Ac-YGRKKRRQRRR-gaba-ILKQSMTLNLADPVGSLKPHLRAKN-NH₂

  • Empirical Formula (Hill Notation) C191H336N70O48S

  • Molecular Weight 4413.22

Purchase

Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
packaging   1 mg in Plastic ampoule (420130-1MG)
color   white
form   lyophilized solid
mfr. no.   Calbiochem
shipped in   wet ice
solubility   DMSO: 100 mg/mL
  acetic acid: 100 mg/mL
storage condition   OK to freeze
  desiccated
  protect from light
purity   ≥95% by HPLC
storage conditions   -20C

Description

Biochem/physiol Actions

Cell permeable: yes

Primary Target
JNK

Product does not compete with ATP.

Reversible: no

General description

A cell-permeable 37 amino acid peptide constructed by fusing the JNK binding domain sequence (δ) (amino acids 33-57) of human c-Jun to the HIV-TAT transduction domain sequence (amino acids 47-57) with a γ-aminobutyric acid (GABA) spacer. Reported to specifically disrupt c-Jun/JNK complex formation and subsequent phosphorylation and activation of c-Jun by JNK in vitro and in intact cells. Also reported to induce apoptosis in HeLa cells. Mode of inhibition is distinct from that of JNK Inhibitor II (SP600125; Cat. No. 420119), so may complement this inhibitor in JNK pathway studies.

A cell-permeable 37-mer peptide constructed by fusing human c-Jun δ domain (amino acids 33-57) sequence with that of HIV-TAT protein transduction domain (amino acids 47-57) via a γ-aminobutyric acid (GABA) spacer. Shown to specifically disrupt c-Jun/JNK complex formation and the subsequent phosphorylation and activation of c-Jun by JNK both in vitro and in intact cells. Since its mode of inhibition is different than that of JNK Inhibitor II (SP600125; Cat. No. 420119), these two inhibitors can complement each other in JNK signaling pathway studies.

Other Notes

Holzberg, D., et al. 2003. J. Biol. Chem.278, 40213.

Packaging

1 mg in Plastic ampoule

Packaged under inert gas

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Carcinogenic / Teratogenic (D)

Physical form

Supplied as a trifluoroacetate salt.

Sequence

Ac-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-gaba-Ile-Leu-Lys-Gln-Ser-Met-Thr-Leu-Asn-Leu-Ala-Asp-Pro-Val-Gly-Ser-Leu-Lys-Pro-His-Leu-Arg-Ala-Lys-Asn-NH₂

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?