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420130 Millipore

JNK Inhibitor III, Cell-Permeable - Calbiochem

The JNK Inhibitor III, Cell-Permeable controls the biological activity of JNK. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Synonym: HIV-TAT₄₇₋₅₇-gaba-c-Junδ₃₃₋₅₇, Ac-YGRKKRRQRRR-gaba-ILKQSMTLNLADPVGSLKPHLRAKN-NH₂, SAPK Inhibitor III, SAPK Inhibitor III, HIV-TAT₄₇₋₅₇-gaba-c-Junδ₃₃₋₅₇, Ac-YGRKKRRQRRR-gaba-ILKQSMTLNLADPVGSLKPHLRAKN-NH₂

  • Empirical Formula (Hill Notation) C191H336N70O48S

  • Molecular Weight 4413.22

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
packaging   1 mg in Plastic ampoule (420130-1MG)
feature productdata solubility   DMSO (100 mg/ml) or Acetic Acid (100 mg/ml)
brand family   Calbiochem
form   Lyophilized white solid
purity   ≥95% by HPLC
storage conditions   Hygroscopic
  Protect from light
  Ok to freeze
shipped in   wet ice
storage conditions   -20C

Description

Biochem/physiol Actions

Cell permeable: yes

Primary Target
JNK

Product does not compete with ATP.

Reversible: no

General description

A cell-permeable 37 amino acid peptide constructed by fusing the JNK binding domain sequence (δ) (amino acids 33-57) of human c-Jun to the HIV-TAT transduction domain sequence (amino acids 47-57) with a γ-aminobutyric acid (GABA) spacer. Reported to specifically disrupt c-Jun/JNK complex formation and subsequent phosphorylation and activation of c-Jun by JNK in vitro and in intact cells. Also reported to induce apoptosis in HeLa cells. Mode of inhibition is distinct from that of JNK Inhibitor II (SP600125; Cat. No. 420119), so may complement this inhibitor in JNK pathway studies.

A cell-permeable 37-mer peptide constructed by fusing human c-Jun δ domain (amino acids 33-57) sequence with that of HIV-TAT protein transduction domain (amino acids 47-57) via a γ-aminobutyric acid (GABA) spacer. Shown to specifically disrupt c-Jun/JNK complex formation and the subsequent phosphorylation and activation of c-Jun by JNK both in vitro and in intact cells. Since its mode of inhibition is different than that of JNK Inhibitor II (SP600125; Cat. No. 420119), these two inhibitors can complement each other in JNK signaling pathway studies.

Other Notes

Holzberg, D., et al. 2003. J. Biol. Chem.278, 40213.

Packaging

Packaged under inert gas

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Warning

Carcinogenic / Teratogenic (D)

Physical form

Supplied as a trifluoroacetate salt.

Sequence

Ac-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-gaba-Ile-Leu-Lys-Gln-Ser-Met-Thr-Leu-Asn-Leu-Ala-Asp-Pro-Val-Gly-Ser-Leu-Lys-Pro-His-Leu-Arg-Ala-Lys-Asn-NH₂

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Protocols & Articles
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