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  • 420297 - InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

420297 Sigma-Aldrich

InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

Synonym: InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

  • CAS Number 97161-97-2

  • Empirical Formula (Hill Notation) C27H21N3O5

  • Molecular Weight 467.47



Related Categories A to C, Biochemicals and Reagents, Calmodulin dependent Protein Kinase (CaM-KK), Cell Biology, Cell Signaling and Neuroscience,
Quality Level   100
assay   ≥95% (HPLC)
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   -20C
shipped in   wet ice
InChI   1S/C27H21N3O5/c1-26-27(33,25(32)34-2)11-18(35-26)29-16-9-5-3-7-13(16)20-21-15(12-28-24(21)31)19-14-8-4-6-10-17(14)30(26)23(19)22(20)29/h3-10,18,33H,11-12H2,1-2H3,(H,28,31)


General description

A cell-permeable protein kinase inhibitor that inhibits CaM kinase II (Ki = 1.8 nM), myosin light chain kinase (Ki = 17 nM), protein kinase A (Ki = 18 nM), protein kinase C (Ki = 25 nM), and protein kinase G (Ki = 20 nM). Also acts as a potent inhibitor (IC50 = 3 nM) of the tyrosine protein kinase activity of the NGF receptor gp 140trk, the product of the trk protooncogene. Also inhibits the kinase activity of its transforming alleles, the trk oncogenes, and of the related neurotrophin receptors gp 145trkB and gp 145trkC. Induces apoptosis and cell cycle arrest by inhibiting Cdc2 and Cdc25c.


100 μg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: yes

Primary Target
CaM kinase II

Product competes with ATP.

Reversible: yes

Target Ki: 1.8 nM, 20 nM, 18 nM, 25 nM, 20 nM, 3 nM, against CaM kinase II, myosin light chain kinase, protein kinase A, protein kinase C, protein kinase G, NGF receptor gp140trk, respectively


Packaged under inert gas


Toxicity: Irritant (B)

Physical form

A 1 mM (100 µg/214 µl) solution of K-252a (Cat. No. 420298) in DMSO.


Following initial thaw aliquot and freeze at (-20°C). Store under an inert atmosphere.

Other Notes

Chin, L.S., et al. 1999. Cancer Invest.17, 391.
Nakanishi, N., et al. 1995. FEBS Lett.368, 411.
Ross, A.H., et al. 1995. J. Neurochem.65, 2748.
Mizuno, K., et al. 1993. FEBS Lett.330, 114.
Pawlowska, Z., et al. 1993. J. Neurochem.60, 678.
Tapley, P., et al. 1992. Oncogene7, 371.
Simpson, D.L., et al. 1991. J. Neurosci. Res.28, 486.
Hashimoto, S. 1988. J. Cell Biol.107, 1531.
Koizuma, S., et al. 1988. J. Neurosci.8, 715.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

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