• USA Home
  • 420297 - InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

EMAIL THIS PAGE TO A FRIEND
420297 Sigma-Aldrich

InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

Synonym: InSolution K-252a, Nocardiopsis sp. - CAS 97161-97-2 - Calbiochem

  • CAS Number 97161-97-2

  • Empirical Formula (Hill Notation) C27H21N3O5

  • Molecular Weight 467.47

Purchase

Properties

Related Categories A to C, Biochemicals and Reagents, Calmodulin dependent Protein Kinase (CaM-KK), Cell Biology, Cell Signaling and Neuroscience,
Quality Level   100
assay   ≥95% (HPLC)
form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   -20C
shipped in   wet ice
InChI   1S/C27H21N3O5/c1-26-27(33,25(32)34-2)11-18(35-26)29-16-9-5-3-7-13(16)20-21-15(12-28-24(21)31)19-14-8-4-6-10-17(14)30(26)23(19)22(20)29/h3-10,18,33H,11-12H2,1-2H3,(H,28,31)
InChI key   KOZFSFOOLUUIGY-UHFFFAOYSA-N

Description

General description

A cell-permeable protein kinase inhibitor that inhibits CaM kinase II (Ki = 1.8 nM), myosin light chain kinase (Ki = 17 nM), protein kinase A (Ki = 18 nM), protein kinase C (Ki = 25 nM), and protein kinase G (Ki = 20 nM). Also acts as a potent inhibitor (IC50 = 3 nM) of the tyrosine protein kinase activity of the NGF receptor gp 140trk, the product of the trk protooncogene. Also inhibits the kinase activity of its transforming alleles, the trk oncogenes, and of the related neurotrophin receptors gp 145trkB and gp 145trkC. Induces apoptosis and cell cycle arrest by inhibiting Cdc2 and Cdc25c.

Packaging

100 μg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: yes

Primary Target
CaM kinase II

Product competes with ATP.

Reversible: yes

Target Ki: 1.8 nM, 20 nM, 18 nM, 25 nM, 20 nM, 3 nM, against CaM kinase II, myosin light chain kinase, protein kinase A, protein kinase C, protein kinase G, NGF receptor gp140trk, respectively

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 1 mM (100 µg/214 µl) solution of K-252a (Cat. No. 420298) in DMSO.

Reconstitution

Following initial thaw aliquot and freeze at (-20°C). Store under an inert atmosphere.

Other Notes

Chin, L.S., et al. 1999. Cancer Invest.17, 391.
Nakanishi, N., et al. 1995. FEBS Lett.368, 411.
Ross, A.H., et al. 1995. J. Neurochem.65, 2748.
Mizuno, K., et al. 1993. FEBS Lett.330, 114.
Pawlowska, Z., et al. 1993. J. Neurochem.60, 678.
Tapley, P., et al. 1992. Oncogene7, 371.
Simpson, D.L., et al. 1991. J. Neurosci. Res.28, 486.
Hashimoto, S. 1988. J. Cell Biol.107, 1531.
Koizuma, S., et al. 1988. J. Neurosci.8, 715.
Kase, H., et al. 1987. Biochem. Biophys. Res. Commun.142, 436.

Safety & Documentation

Safety Information

Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?