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420307 Sigma-Aldrich

Kallikrein, Human Plasma

Native Kallikrein from human plasma. A serine protease that releases bradykinin from kininogen. Present in the plasma as an inactive precursor, prokallikrein.

Synonym: Kallikrein, Human Plasma, Kininogenin, Kininogenase

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Properties

Quality Level   100
assay   ≥95% (SDS-PAGE)
form   liquid
specific activity   ≥15 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   -20C

Description

General description

Native Kallikrein from human plasma. A serine protease that releases bradykinin from kininogen. Present in the plasma as an active precursor, prokallikrein.

Native Kallikrein from human plasma. A serine protease that releases bradykinin from kininogen. Present in the plasma as an inactive precursor, prokallikrein.

Packaging

50 μg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol D-Pro-Phe-Arg-pNA per min at 25°C, pH 8.0.

Physical form

In NaCl and Tris-HCl buffer.

Preparation Note

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Pu, X., et al. 1994. Biochim. Biophys. Acta 1208, 338.
Nagase, H. and Barrett, A.J. 1981. Biochem. J.193, 187.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
Peer-Reviewed Papers
15

References

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