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429705 Sigma-Aldrich

Leptin, Mouse, Recombinant, E. coli

Synonym: βrmOB, βrmOB

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Properties

Related Categories Auxiliary Proteins, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Other, Proteins and Derivatives More...
brand family   Calbiochem
feature productdata solubility   Reconstitute with 20 mM Tris-HCl, pH 8.0 to a final concentration of 1 mg/ml.
form   Lyophilized
impurities   Endotoxin: ≤1.0 EU/µg leptin
purity   ≥97% by SDS-PAGE
shipped in   ambient
storage conditions   = -70C
  Ok to freeze

Description

Biochem/physiol Actions

ED₅₀ = 0.2-1 ng/ml as measured by its ability to induce proliferation of leptin-dependent rOB-R transfected murine BAF3 cells

General description

Recombinant, mouse leptin espressed in E. coli. Leptin was originally identified as a protein product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding leptin have been isolated from human, simian, mouse and rat cells. Human leptin shares ~84% sequence identity with the mouse protein. Human leptin cDNA encodes a 167 amino acid residue protein with a 21 amino acid signal sequence that is cleaved to yield the 146 amino acid mature protein. The expression of leptin mRNA has been shown to be restricted to adipose tissue.



A high-affinity receptor for leptin (OB-R) with homology to gp130 and the G-CSF receptor was subsequently cloned. The OB-R cytoplasmic domain transduces the leptin signal through the JAK-STAT pathway. Although OB-R mRNA was initially shown to be expressed predominantly in the choroid plexus and in the hypothalamus, more recent data also revealed the expression of this receptor in endothelial cells (Ecs). Furthermore, the angiogenic activity of leptin has been demonstrated both in vitro and in vivo, suggesting a physical mechanism whereby leptin-induced angiogenesis may facilitate increased energy expenditure.

Recombinant, mouse leptin espressed in E. coli. Native leptin is a product of the obese (ob) gene that serves as a ligand for the OB receptor (OB-R). Mice with mutations in the ob gene have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. Suppresses insulin secretion by inhibiting activities of Ca2+-dependent PKC isoforms. Reported to reduce hepatic glucose production by blocking phosphoenolpyruvate synthesis.

Other Notes

Anderwald, C., et al. 2002. Mol. Endocrinol.16, 1612.
Ookuma, M., et al. 1998. Diabetes 47, 219.
Campfield, L.A., et al. 1995. Science269, 546.
Halaas, J.L., et al. 1995. Science269, 543.
Pelleymounter, M.A., et al. 1995. Science 269, 540.
Zhang, Y., et al. 1994. Nature 372, 425.

Reconstitution

Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C or -70°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C or -70°C. Avoid freeze/thaw cycles of solutions.

Warning

Standard Handling (A)

Physical form

Lyophilized from a sterile filtered PBS.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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