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444232 Sigma-Aldrich

MMP-9, Dimer, Human Neutrophil

Synonym: Matrix Metalloproteinase 9, Gelatinase B, 92 kDa type IV Collagenase

  •  EC Index Number 3.4.24.35

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
brand family   Calbiochem
form   Liquid
impurities   No other MMP activity detectable
purity   ≥90% by SDS-PAGE
shipped in   wet ice
specific activity   ≥1000 mU/mg protein
storage conditions   = -70C
  Avoid freeze/thaw
  Ok to freeze

Description

General description

Dimeric, native MMP-9 from stimulated human neutrophils. Enzyme should be activated just prior to use.

M.W. 222,000 (non-reducing SDS-PAGE). Note: 1 mU = 1 milliunit.

Other Notes

Olson, M.W. et al. 2000. J. Biol. Chem.275, 2661.
Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.

Warning

Standard Handling (A)

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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