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444234 Sigma-Aldrich

MMP-9-Lipocalin-TIMP-1 Complex, Human Neutrophil

Synonym: Matrix Metalloproteinase 9-Lipocalin-TIMP-1 Complex

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
assay   ≥90% (SDS-PAGE)
form   liquid
specific activity   ≥600 mU/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C
foreign activity   other MMP activity, none detected
shipped in   wet ice

Description

General description

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the Pro-MMP-9/TIMP-1 complex, acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase, with reduced activity.

Native MMP-9-Lipocalin-TIMP-1 complex from stimulated human neutrophils. The ternary MMP-9/lipocalin/TIMP-1 complex behaves like the progelatinase B/TIMP-1 complex in acting as an inhibitor of active MMPs. Following activation, the complex acts as a gelatinase with reduced activity.

Note: 1 mU = 1 milliunit.

Packaging

5 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C, pH 7.0.

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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