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444236 Sigma-Aldrich

Anti-MMP-9 Rabbit pAb

liquid, clone, Calbiochem®

Synonym: Anti-Gelatinase B, Anti-Matrix Metalloproteinase 9

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Properties

Related Categories Alphabetical Index, Antibodies, MF-MN, Primary Antibodies
clone   polyclonal
biological source   rabbit
species reactivity   human, marmoset
form   liquid
shipped in   wet ice
isotype   IgG
Quality Level   100
antibody product type   primary antibodies
does not contain   preservative
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C

Description

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes all forms of MMP-9 under reducing and non-reducing conditions.

Recognizes all forms of MMP-9 under reducing and non-reducing conditions. Exhibits only slight cross-reactivity with MMP-2. Does not cross-react with MMP-1, MMP-3, or MMP-8.

This Anti-MMP-9 Rabbit pAb is validated for use in ELISA, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of MMP-9.

Immunogen

human pro-MMP-9

Application

ELISA (see comments)

Immunoblotting (1:500-1:1000)

Immunofluorescence (1:50-1:250)

Immunohistochemistry (1:40-1:150)

Immunoprecipitation (1:20-1:60)

Physical form

Undiluted serum.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Packaging

500 μL in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Other Notes

Exhibits very slight reactivity with MMP-2. Does not cross-react with collagenases, MMP-1, MMP-3, or MMP-8. This antibody has also been reported to work for ELISA. Variables associated with assay conditions will dictate the proper working dilution.

Kolkenbrock, H., et al. 1996. Biol. Chem.377, 529.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Articles

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