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454849 Sigma-Aldrich

MAP Kinase 1, His•Tag, Human, Recombinant, E. coli

Synonym: ERK1, MAPK3, p44 MAPK

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Cycle Proteins, Antibodies and Reagents, Cell Cycle Regulators, Cell Signaling and Neuroscience More...
Quality Level   100
assay   ≥95% (SDS-PAGE)
form   liquid
specific activity activity   ≥6000 units/mg protein
mfr. no.   Calbiochem®
storage condition   OK to freeze
  avoid repeated freeze/thaw cycles
storage conditions   = -70C
foreign activity   Proteases, none detected
shipped in   wet ice

Description

General description

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of purified, recombinant MAP kinase in vitro with MEK1. Suitable for labeling MAP kinase substrates or for inhibitor screening.

Recombinant, human MAP Kinase 1 fused to a His•Tag sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.

Packaging

2 μg in Plastic ampoule

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.

Physical form

In 100 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.5.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Charest, D.L., et al. 1993. Mol. Cell Biol. 13, 4679.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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