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475981 Sigma-Aldrich

Myosin Light Chain Kinase Inhibitor Peptide 18 - Calbiochem

The Myosin Light Chain Kinase Inhibitor Peptide 18 controls the biological activity of Myosin Light Chain Kinase. This small molecule/inhibitor is primarily used for Phosphorylation & Dephosphorylation applications.

Synonym: H-RKKYKYRRK-NH₂, MLCK Inhibitor Peptide 18, MLCK Inhibitor IV, MLCK Inhibitor Peptide 18, H-RKKYKYRRK-NH₂, MLCK Inhibitor IV, Myosin Light Chain Kinase Inhibitor Peptide 18 - Calbiochem

  • Empirical Formula (Hill Notation) C60H105N23O11

  • Molecular Weight 1324.62

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Myosin Light Chain Kinase (MLCK), Serine/Threonine Kinase Biology,
Quality Level   100
assay   ≥97% (HPLC)
form   lyophilized solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   -20C
color   white
solubility   water: 1 mg/mL
  DMSO: 5 mg/mL
shipped in   wet ice

Description

General description

A highly basic, cell-permable, nonapeptide that acts as a selective myosin light chain kinase (MLCK) inhibitor (IC50 = 50 nM). Does not block calmodulin (CaM) or inhibit the kinase activities of Ca2+/calmodulin kinase II (CaMKII) or protein kinase A (PKA). Inhibition is competitive with respect to substrate (Ki = 52 nM). Displays mixed mode inhibition with respect to ATP.

A highly basic, cell-permeable, nonapeptide that acts as a selective Myosin Light Chain Kinase (MLCK) inhibitor (IC50 = 50 nM). The inhibition is competitive with respect to the peptide substrate (Ki = 52 nM) and displays a mixed mode of inhibition with respect to ATP. Does not interfere with kinase activation by calmodulin (CaM), nor does it inhibit the activities of CaMKII and PKA.

Packaging

5 mg in Plastic ampoule

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Mlck

Product does not compete with ATP.

Reversible: no

Target IC50: 50 nM against MLCK

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Sequence

H-Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Zolotarevsky, Y., et al. 2002. Gastroenterology123, 163.
Lukas, T.J., et al. 1999. J. Med. Chem.42, 910.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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