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NDSB-256 - CAS 81239-45-4 - Calbiochem

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins.

Synonym: Dimethylbenzylammonium Propane Sulfonate, NDSB-256 - CAS 81239-45-4 - Calbiochem

  • CAS Number 81239-45-4

  • Empirical Formula (Hill Notation) C12H19NO3S

  • Molecular Weight 257.35

  •  MDL number MFCD00225018

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Properties

Related Categories Biochemicals and Reagents, Detergents, Non-Detergents Sulfobetaines (NDSB) More...
Quality Level   100
assay   ≥99% (TLC)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
storage conditions   +15C to +30C
color   white
solubility   water: 200 mg/mL

Description

General description

A member of the class of nondetergent sulfobetaines. Unlike CHAPS and other zwittergents, NDSBs are nondenaturing even at 1 M concentrations. NDSBs have been used to improve the efficiency of solubilization of membrane proteins, to isolate nuclear proteins, to chromatographically purify halophilic proteins in low ionic strength buffers, and to crystallize proteins. They are zwitterionic over a wide pH range and do not absorb significantly in the near UV range. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. NDSBs can be easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

A non-detergent sulfobetaine. Reported to prevent protein aggregation and facilitate the renaturation of chemically and thermally denatured proteins. Zwitterionic over a wide pH range. Easily removed by dialysis. At 1 M, NDSB-256 will restore 30% of the enzymatic activity of denatured egg white lysozyme; at 800 mM it will restore 16% of the enzymatic activity of denatured β-galactosidase.

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (15-30°C). Stock solutions are stable for up to 3 months at room temperature.

Other Notes

Goldberg, M.E., et al. 1996. Folding & Design1, 21.

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