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480708 Sigma-Aldrich

α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli

Synonym: α2-3,6-Neuraminidase, Clostridium perfringens, Recombinant, E. coli, Acetylneuraminyl Hydrolase, Sialidase

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Protein & Nucleic Acid Isolation
Quality Level   100
form   liquid
specific activity activity   ≥10 units/mL
  ≥250 units/mg protein
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
foreign activity   N-acetylglucosaminidase, α-fucosidase, α- and β-galactosidase, α- and β-mannosidase, proteases, none detected
shipped in   wet ice

Description

General description

Note: 1 mU = 1 milliunit.

Recombinant, Clostridium perfringens α2-3,6-Neuraminidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal α2,3 and α2,6 unbranched sialic acid residues from complex carbohydrates and glycoproteins. This enzyme does not exhibit activity on α2,8 or branched sialic acids.

Packaging

500 miu in Plastic ampoule

Application

Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 1 µmol of methylumbelliferone from 2ʹ-(4-Methylumbelliferyl)-α-D-N-acetylneuraminic Acid (Cat. No. 474495) per min at 37°C, pH 5.0.

Physical form

In 25 mM NaCl, 20 mM Tris-HCl, pH 7.5.

Other Notes

Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Ohta, Y., et al. 1989. J. Biochem (Tokyo) 106, 1086.

Safety & Documentation

Safety Information

RTECS 
QQ3450000

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