• USA Home
  • 484235 - p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem

EMAIL THIS PAGE TO A FRIEND
484235 Sigma-Aldrich

p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC₅₀ = 3-4 µM).

Synonym: p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem, NBT, Nitro BT

  • CAS Number 298-83-9

  • Empirical Formula (Hill Notation) C40H30Cl2N10O6

  • Molecular Weight 817.64

  •  MDL number MFCD00012159

Purchase

Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Signaling Enzymes, Enzymes, Inhibitors, and Substrates, NOS - Nitric Oxide Inhibitors,
Quality Level   100
assay   ≥98% (TLC)
form   crystalline solid
potency   3-4 μM IC50
mfr. no.   Calbiochem®
storage condition   OK to freeze
  desiccated
  protect from light
storage conditions   +2C to +8C
color   yellow
solubility   methanol: soluble
  water: soluble
shipped in   ambient
InChI   1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
InChI key   FSVCQIDHPKZJSO-UHFFFAOYSA-L

Description

General description

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).

NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).

Packaging

1 g in Glass bottle

250 mg in Glass bottle

Biochem/physiol Actions

Primary Target
NOS

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.

Other Notes

Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis.16, 29.
Libon, C., et al. 1993. J. Leukoc. Biol.53, 93.
Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
Nichols, K., et al. 1992. Neuroscience51, 791.
Nichols, K., et al. 1992. Neuroscience51, 791.

Safety & Documentation

Safety Information

Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?