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507858 Sigma-Aldrich

PANSORBIN Cells

Synonym: Staphylococcus aureus Cells, Staphylococcus aureus Cells

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Properties

Related Categories Antibodies, Supplementary Products More...
brand family   Calbiochem
activity   ≥2.0 mg human IgG/100 mg cells
form   Homogeneous, milky suspension
impurities   Viable cells: ≤2500 cells/ml
shipped in   ambient
storage conditions   +2C to +8C
  Yes

Description

General description

PANSORBIN Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.

Heat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.

Other Notes

Kierszenbaum, F., et al. 1991. Immunology74, 317.
Meikle, P.J., et al. 1991. J. Biol. Chem.266, 22569.
Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
Murakami, H., et al. 1988. Biochem. J.256, 917.
Kessler, S.W. 1975. J. Immunol. 115, 1617.

Warning

Standard Handling (A)

Physical form

Supplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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