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516854 Millipore

Pertussis Toxin, A Protomer, Bordetella pertussis - Calbiochem

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Properties

form   lyophilized
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
impurities   ≤0.1% holotoxin (CHO cell assay)
shipped in   ambient

Description

General description

This product has been discontinued.



Enzymatic component of the holotoxin. Has both NAD-glycohydrolase and ADP-ribosyltransferase activities. Inhibits neutrophil-mediated inflammation in vivo via its ADP-ribosylation activity. Unable to penetrate cells in the absence of the B oligomer.

Enzymatic component of the holotoxin. Has NAD-glycohydrolase activities. Unable to penetrate cells in the absence of the B oligomer.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Has both NAD-glycohydrolase and ADP-ribosyltransferase activities

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Toxic (F)

Physical form

Lyophilized from 10 mM Tris, 100 µM EDTA, 0.04% CHAPS, pH 8.0.

Reconstitution

Following reconstitution, refrigerate (4°C). Stock solutions, prepared from high ionic strength buffers, are stable for up to 3 months at 4°C.

Reconstitute with 500 µl sterile dH₂O or high ionic buffer.

Analysis Note

Single major band by SDS-PAGE

Other Notes

Brito, G.A., et al. 1997. Infect. Immun. 65, 1114.
Gill, D.M., and Coburn, J., 1988. Biochim. Biophys. Acta954, 65.
Kaslow, H.R., and Lesikar, D.D., 1987. Biochemistry26, 4397.
Burns, D.L., and Manclark, C.R., 1986. J. Biol. Chem.261, 4324.
Moss, J., et al. 1983. J. Biol. Chem.258, 11879.
Tamura, M., et al. 1982. Biochemistry21, 5516.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Safety & Documentation

Safety Information

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