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529659 Sigma-Aldrich

Pro-survival Compound, DDD00033325 - Calbiochem

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background with equal or higher potency than Y-27632.

Synonym: DDD00033325, Pro-survival Compound, DDD00033325 - Calbiochem

  • Empirical Formula (Hill Notation) C17H19N5

  • Molecular Weight 293.37

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Properties

Quality Level   100
assay   ≥95% (HPLC)
form   syrup
mfr. no.   Calbiochem®
storage condition   OK to freeze
  protect from light
storage conditions   -20C
color   yellow
solubility   DMSO: 100 mg/mL
shipped in   wet ice

Description

General description

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC50 = 3.9 µM), with equal or higher potency than Y-27632 ((Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 µM. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (>83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 µM. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 µM than Y-27632 without marked adverse effects for up to 120 hours.

A cell-permeable, pyrrolidine small molecule that promotes single human embryonic stem cell survival up to 20-fold over background (EC50 = 3.9 µM), with equal or higher potency than Y-27632 ((Cat. No. 688000) in 5 cell lines (SA461, SA181, SA167, SA121 and SA002) tested at 10 µM. An in vitro high-content screening assay, which profiles a panel of more than 70 protein kinases, indicates that this small molecule is more selective than Y-27632 and only displays an inhibitory effect toward ROCK2 (> 83% inhibition, ROCK1 was not included in the panel) and PRK2 (76% inhibition) at 10 µM. Furthermore, it exhibits less cytotoxicity at a greater concentration of 30 µM than Y-27632 without marked adverse effects for up to 120 hours.

Packaging

10 mg in Glass bottle

Packaged under inert gas

Warning

Toxicity: Regulatory Review (Z)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Andrews, P.D., et al. 2010. Biochem. J.432, 21.

Safety & Documentation

Safety Information

Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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