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537088 Millipore

PRONASE Protease, Streptomyces griseus, Nuclease-Free

Widely used broad-spectrum protease mixture

Synonym: PRONASE Protease, Streptomyces griseus, Nuclease-Free

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Properties

Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Pronase E, Protein & Nucleic Acid Isolation,
form   lyophilized
specific activity   ≥70,000 proteolytic units/g dry wt
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C
solubility   water: 10 mg/mL
foreign activity   Nuclease, none detected
shipped in   ambient

Description

General description

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at neutral pH, but stable over wide range of pH and temperature. Note: 1KU = 1000 units.

A non-specific protease liquefies mucins and digests proteins to free amino acids. Can be used to isolate living chondrocytes. Most active at pH 7.0 but stable over wide ranges of pH and temperature. Has an optimal pH of 7.5.

Note: 1 KU = 1000 units.

Packaging

10, 100, 5, 50 ku in Plastic ampoule

25 ku in Glass bottle

Warning

Toxicity: Harmful (C)

Unit Definition

One unit is defined as the amount of enzyme that will liberate a digestion product equivalent to 25 µg of tyrosine per min at 40°C, pH 7.5.

Reconstitution

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

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Signal word 
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Hazard statements 
Target organs 
Respiratory system
WGK Germany 
WGK 2
Protocols & Articles
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