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539124 Millipore

Protease Arrest Reagent - Calbiochem

An optimized concentration of various reversible and irreversible inhibitors to inhibit serine, cysteine, and calpain proteases.

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Properties

form   liquid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   +2C to +8C

Description

General description

An optimized concentration of various reversible and irreversible inhibitors to inhibit serine, cysteine, and calpain proteases. Suitable for the protection of proteins purified from animal tissues, plant tissues, yeast, and bacteria. EDTA is also provided separately to inhibit metalloproteinases. Protease Arrest Reagent is provided as a 100X solution that when diluted in extraction buffer at pH 7.0-8.0 inhibits 95-98% of protease activity. Supplied with a data sheet.

An optimized concentration of various reversible and irreversible inhibitors to inhibit serine, cysteine, and calpain proteases.

Protease Arrest Reagent provides optimized concentration of the various inhibitors, and exhibits excellent inhibitory activity towards various proteases. It is suitable for protection of proteins during purification. It can be used with animal tissues, plant tissues, yeast and bacteria. Protease Arrest Reagent contains a blend of both irreversible and reversible inhibitors for serine, and cysteine proteases and for calpains. A vial of EDTA is also provided to inhibit metalloproteases. Please note, however, that some proteins may require divalent cations like Ca2+, Mg2+ or Mn2+ for their activity, as the presence of EDTA may be detrimental to their enzymatic activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The EDTA solution may be added in the extraction buffer or lysate as needed. Protease Arrest Reagent at 1X concentration in extraction buffer at pH 7-8 inhibits over 95% of protease activities. Each set contains the following components



• Protease-Arrest Reagent (100X) 2 ml

• EDTA (0.5 M) 2 ml

Packaging

1 set in Plastic ampoule

Biochem/physiol Actions

Cell permeable: no

Primary Target
Serine, cysteine, and calpain proteases

Warning

Toxicity: Irritant (B)

Reconstitution

Following addition to buffers or extracts this product is stable for up to 2 weeks at 4°C or for up to 3 months at -20°C.

Safety & Documentation

Safety Information

Protocols & Articles
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