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539305 Sigma-Aldrich

Protein G, Alkaline Phosphatase Conjugate



Quality Level   100
form   liquid
contains   ≤0.1% sodium azide as preservative
mfr. no.   Calbiochem®
storage condition   do not freeze
storage conditions   +2C to +8C
shipped in   wet ice


General description

Protein G conjugated to alkaline phosphatase. Does not react with IgA, IgE, or IgM.

Recombinant protein G, a cell-surface protein of Streptococcus species, binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economical means to isolate, detect and purify antibodies produced by many animals. It is especially valued because of its ability to react with human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e., bovine goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE, and IgM. Useful for detection of primary antibodies by various immunoassay methods at an average dilution of 1:2500.


500 μg in Plastic ampoule


Direct Immunoassay (1:2500)


Please refer to vial label for lot-specific concentration.


Toxicity: Standard Handling (A)

Physical form

In 50 mM Tris buffer, 1 mM MgCl₂, 0.1 mM ZnCl₂, 1% BSA, 0.1% sodium azide, pH 7.6.

Analysis Note

Single band at 33.8 kDa by SDS-PAGE

Other Notes

p-Nitrophenyl phosphate, 1.0 mg/ml in 10% diethanolamine, pH 9.8, 1 mM MgCl2 at 25°C, was used as substrate to test immunoenzyme reactivity. Variables associated with assay conditions will dictate the proper working dilution.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
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