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  • 552095 - R-Spondin 4, Mouse, Recombinant

552095 Sigma-Aldrich

R-Spondin 4, Mouse, Recombinant

Synonym: Cristin 4



Quality Level   100
assay   ≥95% (SDS-PAGE)
form   solid
mfr. no.   Calbiochem®
storage condition   OK to freeze
storage conditions   -20C
impurities   <1.0 EU/μg protein Endotoxin
shipped in   ambient


General description

Recombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.


25 μg in Glass bottle

Biochem/physiol Actions

ED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.


Toxicity: Standard Handling (A)

Physical form

Lyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.


Reconstitute to 200 µg/ml in PBS.

Other Notes

Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ.50, 229.
Ishii, Y., et al. 2008. J. Invest. Dermatol.128, 867.
Kim, K.A., et al. 2008. Mol. Biol. Cell19, 2588.
Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA104 14700.
Nam, J.S., et al. 2007. Gene Expr. Patterns7, 306.
Pemberton, T.J., et al. 2007. Dev. Dyn.236, 2245.
Bergmann, C., et al. 2006. Am. J. Hum. Genet.79, 1105.
Blaydon, D.C., et al. 2006. Nat. Genet.38, 1245.
Nam, J.S., et al. 2006. J. Biol. Chem.281, 13247.

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